SDS is a detergent used throughout protein work. A 10% w/v stock means 10 g per 100 mL. SDS dust is an irritant, so weigh it carefully.
Scale this recipe. Recipe makes 100 mL. Make instead:
Ingredient amounts below update automatically.
Ingredients
| Component | Amount | Note |
|---|---|---|
| Sodium dodecyl sulfate (SDS) | 10 g | 10% w/v |
| Deionised water | 80 mL | to final volume |
Method
- Wearing a mask to avoid inhaling the fine powder, add the SDS to about 80 mL of deionised water (per 100 mL final).
- Stir gently and warm to about 60 °C if needed to dissolve; avoid vigorous frothing.
- Once dissolved and cooled, make up to the final volume with deionised water.
- Store at room temperature; do not refrigerate, as SDS precipitates in the cold.
Notes & safety
SDS precipitates below about 15 °C. If the stock clouds in a cool room, warm and stir to redissolve. Always weigh SDS in a way that contains the dust.
Consult the Safety Data Sheet for each reagent and follow your institution's protocols. Wear appropriate PPE.
Related guides: Percent Concentration Solutions · How to Prepare a Solution of a …
Equipment for this prep
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