TBE has a higher buffering capacity than TAE and is preferred for longer runs and smaller DNA fragments. Keep a 10× stock and dilute to 1× (or 0.5×) for use.
Scale this recipe. Recipe makes 1 L. Make instead:
Ingredient amounts below update automatically.
Ingredients
| Component | Amount | Note |
|---|---|---|
| Tris base | 108 g | MW 121.14 g/mol |
| Boric acid | 55 g | MW 61.83 g/mol |
| 0.5 M EDTA (pH 8.0) | 40 mL | see the EDTA recipe |
| Deionised water | 800 mL | to final volume |
Method
- Dissolve the Tris base and boric acid in about 800 mL of deionised water.
- Add the 0.5 M EDTA (pH 8.0).
- Make up to the final volume with deionised water and mix.
- Store at room temperature. Dilute 1:10 for 1× working buffer (or 1:20 for 0.5×).
Notes & safety
Concentrated TBE can form a precipitate on long storage; warm gently and mix to redissolve, or filter before use. 0.5× TBE is common for gel running to reduce heating.
Consult the Safety Data Sheet for each reagent and follow your institution's protocols. Wear appropriate PPE.
Related guides: Dilution and Serial Dilution Ex… · How to Make a Buffer Solution
Equipment for this prep
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